Abstract
Surface modification of nanocarriers offers the possibility of targeted drug delivery, which is of major interest in modern pharmaceutical science. Click-chemistry affords an easy and fast way to modify the surface with targeting structures under mild reaction conditions. Here we describe our current method for the post-preparational surface modification of multifunctional sterically stabilized (stealth) liposomes via copper-catalyzed azide–alkyne cycloaddition (CuAAC) and inverse electron demand Diels-Alder norbornene–tetrazine cycloaddition (IEDDA). We emphasize the use of these in a one-pot orthogonal reaction for deep investigation on stability and targeting of nanocarriers. As the production of clickable amphiphilic polymers is a limiting factor in most cases, we also describe our nanocarrier preparation technique called dual centrifugation, which enables the formulation of liposomes on a single-digit milligram scale of total lipid mass.
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Acknowledgements
The Rotanta 400 dual centrifuge prototype was kindly provided by Andreas Hettich GmbH, Tuttlingen, Germany. The authors would like to thank the collaborative research center SFB 1066 (Project A7) by the German Research Foundation (DFG).
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Voigt, M., Fritz, T., Worm, M., Frey, H., Helm, M. (2019). Surface Modification of Nanoparticles and Nanovesicles via Click-Chemistry. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_16
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DOI: https://doi.org/10.1007/978-1-4939-9516-5_16
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