Abstract
Ultraviolet resonance Raman (UVRR) spectroscopy is a vibrational technique that reveals structures and dynamics of biological macromolecules without the use of extrinsic labels. By tuning the Raman excitation wavelength to the deep UV region (e.g., 228 nm), Raman signal from tryptophan and tyrosine residues are selectively enhanced, allowing for the study of these functionally relevant amino acids in lipid and aqueous environments. In this chapter, we present methods on the UVRR data acquisition and analysis of the tryptophan vibrational modes of a model β-barrel membrane protein, OmpA, in folded and unfolded conformations.
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Acknowledgments
D.K.A. acknowledges the UCSD Molecular Biophysics Training NIH Grant T32 GM008326 for funding support of this work.
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Asamoto, D.K., Kim, J.E. (2019). UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics. In: Kleinschmidt, J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9512-7_14
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DOI: https://doi.org/10.1007/978-1-4939-9512-7_14
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