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DNA-Encoded Macrocyclic Peptide Library

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Cyclic Peptide Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2001))

Abstract

DNA-encoded library technology (ELT) is a cutting-edge enabling technology platform for drug discovery. Here we describe how to design and synthesize a macrocyclic DNA-encoded library; how to perform selection, sequencing, and data analysis to identify potential active peptides; and how to synthesize off-DNA peptides to confirm activity. This approach provides an effective tool for pharmaceutical research based on peptides.

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Author information

Authors and Affiliations

  1. GlaxoSmithKline, Cambridge, MA, USA

    Zhengrong Zhu, Alex Shaginian, La Shadric C. Grady, Christopher P. Davie & Kenneth Lind

  2. GlaxoSmithKline, Stevenage, UK

    Sandeep Pal, Praew Thansandote & Graham L. Simpson

Authors
  1. Zhengrong Zhu
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  2. Alex Shaginian
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  3. La Shadric C. Grady
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  4. Christopher P. Davie
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  5. Kenneth Lind
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  6. Sandeep Pal
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  7. Praew Thansandote
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  8. Graham L. Simpson
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Corresponding author

Correspondence to Zhengrong Zhu .

Editor information

Editors and Affiliations

  1. Hit Discovery and Optimization, Pfizer R&D, Groton, CT, USA

    Gilles Goetz

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Zhu, Z. et al. (2019). DNA-Encoded Macrocyclic Peptide Library. In: Goetz, G. (eds) Cyclic Peptide Design. Methods in Molecular Biology, vol 2001. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9504-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9504-2_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9503-5

  • Online ISBN: 978-1-4939-9504-2

  • eBook Packages: Springer Protocols

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