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Telomere and G-Quadruplex Colocalization Analysis by Immunofluorescence Fluorescence In Situ Hybridization (IF-FISH)

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Book cover DNA Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1999))

Abstract

Four-stranded G-quadruplexes consists of tracks of guanines that are stabilized by Hoogsteen base pairing. The formation of G-quadruplexes in genomic DNA contribute to numerous biological processes in vivo, including replication, transcription, and telomere maintenance. Here, we present a detailed method to detect the colocalization of G-quadruplexes with telomeres in vivo, using the BG4 antibody developed from Dr. Shankar Balasubramanian’s lab.

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Acknowledgments

We thank Dr. Shankar Balasubramanian (University of Cambridge, UK) for providing BG4 antibody (plasmid). This work was supported by Ministry of Science and Technology (2017YFC1001904), National Natural Science Foundation of China (91649102, 31771520, 21177091, 21647008, 31471293, 81501386, 81671054, 81771135), the Key Project of Tianjin Research Program of Application Foundation and Advanced Technology (15JCZDJC35100).

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Authors and Affiliations

  1. Department of Genetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, People’s Republic of China

    Miaomiao Zhang, Rui Liu & Feng Wang

Authors
  1. Miaomiao Zhang
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  2. Rui Liu
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  3. Feng Wang
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Corresponding author

Correspondence to Feng Wang .

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Editors and Affiliations

  1. Indiana University – Purdue University, Indianapolis, IN, USA

    Lata Balakrishnan

  2. Department of Biological Sciences, University of South Carolina, Columbia, SC, USA

    Jason A. Stewart

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Zhang, M., Liu, R., Wang, F. (2019). Telomere and G-Quadruplex Colocalization Analysis by Immunofluorescence Fluorescence In Situ Hybridization (IF-FISH). In: Balakrishnan, L., Stewart, J. (eds) DNA Repair. Methods in Molecular Biology, vol 1999. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9500-4_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9500-4_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9499-1

  • Online ISBN: 978-1-4939-9500-4

  • eBook Packages: Springer Protocols

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