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Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts

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DNA Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1999))

Abstract

Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein–DNA interactions with great spatial and temporal detail.

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Acknowledgments

This work was supported by NIH grant R35GM119512 to D.T.L. and T32 CA193201 to K.B.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA

    Kelly B. Wolfe & David T. Long

Authors
  1. Kelly B. Wolfe
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  2. David T. Long
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Corresponding author

Correspondence to David T. Long .

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Editors and Affiliations

  1. Indiana University – Purdue University, Indianapolis, IN, USA

    Lata Balakrishnan

  2. Department of Biological Sciences, University of South Carolina, Columbia, SC, USA

    Jason A. Stewart

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Wolfe, K.B., Long, D.T. (2019). Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts. In: Balakrishnan, L., Stewart, J. (eds) DNA Repair. Methods in Molecular Biology, vol 1999. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9500-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9500-4_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9499-1

  • Online ISBN: 978-1-4939-9500-4

  • eBook Packages: Springer Protocols

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