Abstract
The composition of Neisseria peptidoglycan has been of scientific interest for over four decades. Initial investigations focused on discovering the mechanisms causing rising rates of antibiotic resistance in N. gonorrhoeae by determining differences in peptidoglycan composition in penicillin susceptible and resistant strains. The discovery that cytotoxic peptidoglycan fragments are also released by Neisseria furthered the interest in peptidoglycan composition. This method describes the purification, enzymatic degradation, and separation of peptidoglycan fragments by high-performance liquid chromatography (HPLC). It also describes the preparation of samples so that they can be positively identified by mass spectrometry.
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This work was supported by NIH grant R01AI097157.
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Schaub, R.E., Dillard, J.P. (2019). Peptidoglycan Composition in Neisseria. In: Christodoulides, M. (eds) Neisseria gonorrhoeae. Methods in Molecular Biology, vol 1997. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9496-0_8
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DOI: https://doi.org/10.1007/978-1-4939-9496-0_8
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