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RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells

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The ESCRT Complexes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1998))

Abstract

Specific depletion of proteins from cultured cells using RNA interference (RNAi) has been a useful technique in assessing protein function for many years. RNAi allows the degradation of specific, targeted mRNA, allowing the effects of protein depletion on cellular processes to be examined. Here, I present a protocol for the depletion of proteins from cultured HeLa cells and list specific reagents and considerations for targeting the endosomal sorting complexes required for transport (ESCRT).

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Acknowledgments

I would like to thank Dr. Paul Pryor and Prof. Paul Luzio for critical reading of the manuscript.

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Authors and Affiliations

  1. Division of Biosciences, Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK

    Katherine Bowers

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  1. Katherine Bowers
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Correspondence to Katherine Bowers .

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Editors and Affiliations

  1. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Emmanuel Culetto

  2. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Renaud Legouis

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Bowers, K. (2019). RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells. In: Culetto, E., Legouis, R. (eds) The ESCRT Complexes. Methods in Molecular Biology, vol 1998. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9492-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9492-2_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9491-5

  • Online ISBN: 978-1-4939-9492-2

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