Abstract
Members of the archaeal domain of life that lack homologs of actin and tubulin divide by binary fission in a process that is dependent upon orthologs of eukaryotic ESCRT components. Many of these archaeal organisms are hyperthermophilic acidophiles with unique cell wall structures, which create technical challenges for performing traditional cell biological techniques. Here, we describe the “baby machine” method for synchronizing microorganisms at high temperatures in order to study cell cycle-related processes. We also provide details for fixing, permeabilizing, and staining archaeal cells and ESCRT assemblies for observation by light microscopy.
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References
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Acknowledgments
S.D.B. and R.Y.S. are funded by the College of Arts and Sciences, Indiana University, S.D.B.’s lab is funded by grant R01 GM125579-01 from the National Institutes of Health, I.G.D. was supported by the Australian Research Council (FT160100010).
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Samson, R.Y., Duggin, I.G., Bell, S.D. (2019). Analysis of the Archaeal ESCRT Apparatus. In: Culetto, E., Legouis, R. (eds) The ESCRT Complexes. Methods in Molecular Biology, vol 1998. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9492-2_1
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DOI: https://doi.org/10.1007/978-1-4939-9492-2_1
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