Abstract
The separation and analysis of protein–lipid complexes has proven to be challenging due to the harsh conditions required by conventional methods of protein or lipid isolation, which disrupt the fine forces that govern the interactions between lipid head groups and protein side chains. The method described in this publication presents an alternative for the separation of protein–lipid complexes while maintaining the integrity of their interactions. The method exploits the specific electrophoretic forces that are unique to the geometry of the capillary system and allows purification of intact complexes and the systematic analysis of its constituents. This technique is specifically applied for the separation of native protein-lipid complexes found in the central nervous system.
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Reference
Saliba A-E, Vonkova I, Gavin A-C (2015) The systematic analysis of protein-lipid interactions comes of age.(Technology and techniques—innovation)(report). Nat Rev Mol Cell Biol 16(12):753. https://doi.org/10.1038/nrm4080
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Valdivia, A.O., Myer, C., Suarez, M.F., Bhattacharya, S.K. (2019). Protein–Lipid Complex Separation Utilizing a Capillary Electrophoresis System. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_9
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DOI: https://doi.org/10.1007/978-1-4939-9488-5_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9487-8
Online ISBN: 978-1-4939-9488-5
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