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Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS

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Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Pyridine nucleotides which include NAD+, NADH, NADP, and NADPH play vital roles in many different biological processes. These metabolites can be accurately quantified in a wide variety of biological samples using LC-MS/MS. The quality and precision of these measurements was enhanced using heavy isotope-labeled internal standards and carefully crafted protocols for sample processing.

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Acknowledgment

This work was supported by an NIH Common Fund grant (NIHU24 DK097209) awarded to the Southeast Center of Integrated Metabolomics (SECIM), a partnership between the University of Florida and the Sanford Burnham Prebys Medical Discovery Institute in Orlando. The capability to perform the assays described in this methods chapter was transferred to the Translational Research Institute for Metabolism and Diabetes at Advent Health (Orlando, FL).

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Author notes

  1. Chris Petucci

    Present address: Department of Medicine, Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

  2. Nidhi Kapoor & Stephen J. Gardell

    Present address: Translational Research Institute for Metabolism and Diabetes, Advent Health, Orlando, FL, USA

Authors and Affiliations

  1. Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA

    Chris Petucci, Jeffrey A. Culver, Nidhi Kapoor & Stephen J. Gardell

  2. Southeast Center for Integrated Metabolomics (SECIM), Gainesville, FL, USA

    Chris Petucci, Jeffrey A. Culver, Nidhi Kapoor & Stephen J. Gardell

  3. Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA

    E. Hampton Sessions & Daniela Divlianska

Authors
  1. Chris Petucci
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  2. Jeffrey A. Culver
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  3. Nidhi Kapoor
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  4. E. Hampton Sessions
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  5. Daniela Divlianska
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  6. Stephen J. Gardell
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Corresponding author

Correspondence to Stephen J. Gardell .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Petucci, C., Culver, J.A., Kapoor, N., Sessions, E.H., Divlianska, D., Gardell, S.J. (2019). Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

  • eBook Packages: Springer Protocols

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