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Metabolomics Analyses from Tissues in Parkinson’s Disease

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Book cover Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Metabolomics has been successfully applied to study neurological and neurodegenerative disorders including Parkinson’s disease for (1) the identification of potential biomarkers of onset and disease progression; (2) the identification of novel mechanisms of disease progression; and (3) the assessment of treatment prognosis and outcome. Reproducible and efficient extraction of metabolites is imperative to the success of any metabolomics investigation. Unlike other omics techniques, the composition of the metabolome can be negatively impacted by the preparation, processing, and handling of these samples. The proper choice of data collection, preprocessing, and processing protocols is similarly important to the design of an effective metabolomics experiment. Likewise, the correct application of univariate and multivariate statistical methods is essential for providing biologically relevant insights. In this chapter, we have outlined a detailed metabolomics workflow that addresses all of these issues. A step-by-step protocol from the preparation of neuronal cells and metabolomic tissue samples to their metabolic analyses using nuclear magnetic resonance, mass spectrometry, and chemometrics is presented.

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Acknowledgments

This material is based upon work supported by the National Science Foundation under Grant Number 1660921. This work was supported in part by funding from the Redox Biology Center (P30 GM103335, NIGMS) and the Nebraska Center for Integrated Biomolecular Communication (P20 GM113126, NIGMS). The research was performed in facilities renovated with support from the National Institutes of Health (RR015468-01). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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Author notes

  1. Fatema Bhinderwala, Shulei Lei and Jade Woods contributed equally to this chapter.

Authors and Affiliations

  1. Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA

    Fatema Bhinderwala, Shulei Lei, Jade Woods, Darrell D. Marshall, Eli Riekeberg, Aline De Lima Leite, Martha Morton, Eric D. Dodds & Robert Powers

  2. School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA

    Jordan Rose & Rodrigo Franco

  3. Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA

    Jordan Rose, Rodrigo Franco & Robert Powers

  4. Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE, USA

    Aline De Lima Leite, Martha Morton, Eric D. Dodds & Robert Powers

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  1. Fatema Bhinderwala
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  2. Shulei Lei
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  3. Jade Woods
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  5. Darrell D. Marshall
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  7. Aline De Lima Leite
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  9. Eric D. Dodds
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  10. Rodrigo Franco
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  11. Robert Powers
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Correspondence to Rodrigo Franco or Robert Powers .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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Bhinderwala, F. et al. (2019). Metabolomics Analyses from Tissues in Parkinson’s Disease. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_19

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