Skip to main content
SpringerLink
Log in

Assessment of Transport of Lipid Metabolites Within Trabecular Meshwork Cells

  • Protocol
  • First Online:
Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

  • 1977 Accesses

Abstract

Lipids from trabecular meshwork (TM) cells are of particular interest to ophthalmological researchers as a therapeutic target for lowering intraocular pressure (IOP) in glaucomatous eyes. Fluorescence-based lipid transport assays (FBLTA) and immunocytochemistry (ICC) are dynamic fluorescence analysis techniques that allow for quantitative and qualitative comparisons, respectively, between multiple samples. Here we describe methods for FBLTA, ICC, and mass spectroscopy designed to measure the kinetics and localization of lipid metabolites within the trabecular meshwork.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. VanHook AM (2009) Lipids link membrane molecules. Sci Signal 2(100):ec390

    Google Scholar 

  2. Meer GV, Voelker DR, Feigenson GW (2008) Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol 9(2):112–124

    Article  Google Scholar 

  3. Hankins HM, Baldridge RD, Xu P, Graham TR (2014) Role of flippases, scramblases and transfer proteins in phosphatidylserine subcellular distribution. Traffic 16(1):35–47

    Article  Google Scholar 

  4. Bell RM, Ballas LM, Coleman RA (1981) Lipid Topogenesis. J Lipid Res 3:391–403

    Google Scholar 

  5. Ikeda M, Kihara A, Igarashi Y (2006) Lipid asymmetry of the eukaryotic plasma membrane: functions and related enzymes. Biol Pharm Bull 29(8):1542–1546

    Article  CAS  Google Scholar 

  6. Graham TR (2004) Flippases and vesicle-mediated protein transport. Trends Cell Biol 14(12):670–677

    Article  CAS  Google Scholar 

  7. Daleke DL (2002) Regulation of transbilayer plasma membrane phospholipid asymmetry. J Lipid Res 44(2):233–242

    Article  Google Scholar 

  8. Sahu SK, Gummadi SN, Manoj N, Aradhyam GK (2007) Phospholipid scramblases: an overview. Arch Biochem Biophys 462(1):103–114

    Article  CAS  Google Scholar 

  9. Marks DL, Singh RD, Choudhury A, Wheatley CL, Pagano RE (2005) Use of fluorescent sphingolipid analogs to study lipid transport along the endocytic pathway. Methods 36(2):186–195

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso & Sanjoy K. Bhattacharya

Authors
  1. Daphne Toglia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephanie Dancausse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karthik Kalahasty
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bryan Alfonso
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanjoy K. Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sanjoy K. Bhattacharya .

Editor information

Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Toglia, D., Dancausse, S., Kalahasty, K., Alfonso, B., Bhattacharya, S.K. (2019). Assessment of Transport of Lipid Metabolites Within Trabecular Meshwork Cells. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation