Abstract
Enzymes exist in all biological systems to catalyze vital biochemical reactions. The reactivity of an enzyme and the extent of its influence on product formation can give insight to understanding the physiological changes that can take place. The enzyme HSD17B7, involved in the cholesterol biosynthesis pathway, may play a role in influencing underlying changes during transition of disease, specifically in eyes at normal state to eyes that have glaucoma. In this work, we present a method to test the enzymatic activity level of HSD17B7 between normal and glaucomatous optic nerves to assess whether enzymatic upregulation of cholesterol biosynthesis may play a role in glaucoma.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ohnesorg T, Keller B, Hrabe de Angelis M, Adamski J (2006) Transcriptional regulation of human and murine 17β-hydroxysteroid dehydrogenase type-7 confers its participation in cholesterol biosynthesis. J Mol Endocrinol 37(1):185–197
Krazeisen A, Breitling R, Imai K, Fritz S, Moller G, Adamski J (1999) Determination of cDNA, gene structure and chromosomal localization of the novel human 17β-hydroxysteroid dehydrogenase type 7. FEBS Lett 460(2):373–379
Jokela H, Rantakari P, Lamminen T, Strauss L, Ola R, Mutka AL, Gylling H, Miettinen T, Pakarinen P, Sainio K, Poutanen M (2010) Hydroxysteroid (17β) dehydrogenase 7 activity is essential for fetal de novo cholesterol synthesis and for neuroectodermal survival and cardiovascular differentiation in early mouse embryos. Endocrinology 151(4):1884–1892
Marks LS (2004) 5α-reductase: history and clinical importance. Rev Urol 6(Suppl 9):S11–S21
Doyle RM, McDowell D (2018) Thermo Fisher Scientific. Which LC-MS/MS platform is most appropriate for the quantitative analysis of steroids in serum for clinical research? https://assets.thermofisher.com/TFS-Assets/CMD/posters/po-65264-lc-ms-quant-analysis-steroids-serum-asms2018-po65264-en.pdf
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Wu, C., Ton, NS., Suarez, M.F., Piqueras, M., Bhattacharya, S.K. (2019). HSD18B7 Enzyme Assay Technique Using a Triple Quadrupole Mass Spectrometer. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9488-5_13
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9487-8
Online ISBN: 978-1-4939-9488-5
eBook Packages: Springer Protocols