Abstract
Although most algal biofuel research has focused on microalgae, macroalgae are also potential sources of lipid for the production of biodiesel and other liquid fuels. Reliable, accurate methods for assessing the lipid composition of biomass are essential for the development of macroalgae in this area. The conventional methods most commonly used to evaluate lipid composition, such as those of Bligh and Dyer and Folch, do not provide complete extraction of lipids in photosynthetic cells/tissues and therefore do not provide an accurate accounting of lipid production. Here we present a 2-EE lipid extraction protocol, a method which has been demonstrated to be superior to conventional lipid extraction methods for microalgae, adapted for use with macroalgae.
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References
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639
Quinn JC, Davis R (2015) The potentials and challenges of algae based biofuels: a review of the techno-economic, life cycle, and resource assessment modeling. Bioresour Technol 184:444–452
Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, Smith AG (2010) Biodiesel from algae: challenges and prospects. Curr Opin Biotechnol 21(3):277–286
Magnusson M, Mata L, de Nys R, Paul NA (2014) Biomass, lipid and fatty acid production in large-scale cultures of the marine macroalga Derbesia tenuissima (Chlorophyta). Mar Biotechnol 16:456–464
Xu X, Kim JY, Oh YR, Park JM (2014) Production of biodiesel from carbon sources of macroalgae, Laminaria japonica. Bioresour Technol 169:455–461
Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Bligh E, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Jones J, Manning S, Montoya M, Keller K, Poenie M (2012) Extraction of algal lipids and their analysis by HPLC and mass spectrometry. J Am Oil Chem Soc 89:1371–1381
Acknowledgments
This work was supported by NSF grant DBI-1560772 and DOE grant DE-1162-1511.
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Nobles, D.R., Manning, S.R. (2019). Extraction and Characterization of Lipids from Macroalgae. In: Balan, V. (eds) Microbial Lipid Production. Methods in Molecular Biology, vol 1995. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9484-7_7
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DOI: https://doi.org/10.1007/978-1-4939-9484-7_7
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9484-7
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