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Microbial Lipid Production pp 121–129Cite as

Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1995))

Abstract

Laboratory analytical techniques employed for triglyceride quantification in oleaginous biomass (e.g., microalgae and oilseeds) involve multiple steps and typically require use of volatile organic solvents. Here we describe a single-step approach for measurement of triglycerides using thermogravimetry (TG). We have observed that triglycerides undergo complete volatilization over a narrow temperature interval of 370–450 °C, with negligible solid residue under inert atmosphere, whereas other constituents of oleaginous biomass (such as proteins and carbohydrates) primarily degrade below 350 °C. As a result, triglyceride content of biomass can be estimated using TG by determining the mass loss of the sample in the temperature interval of 370–450 °C.

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Acknowledgments

This work was supported by (1) the US Department of Energy Bioenergy Technologies Office (award# DE-EE0005993) and (2) the National Science Foundation through the Sustainable Energy Pathways Program (award# CHE-1230609)

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Authors and Affiliations

  1. Suganit Bio-Renewables LLC, Toledo, OH, USA

    Balakrishna Maddi

  2. Department of Chemical Engineering, The University of Toledo, Toledo, OH, USA

    Agasteswar Vadlamani, Sridhar Viamajala & Sasidhar Varanasi

Authors
  1. Balakrishna Maddi
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  2. Agasteswar Vadlamani
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  3. Sridhar Viamajala
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  4. Sasidhar Varanasi
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Corresponding author

Correspondence to Sridhar Viamajala .

Editor information

Editors and Affiliations

  1. Department of Engineering Technology, Biotechnology Program College of Technology, University of Houston, Houston, TX, USA

    Venkatesh Balan

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Maddi, B., Vadlamani, A., Viamajala, S., Varanasi, S. (2019). Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry. In: Balan, V. (eds) Microbial Lipid Production. Methods in Molecular Biology, vol 1995. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9484-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9484-7_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9483-0

  • Online ISBN: 978-1-4939-9484-7

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