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Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm

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Book cover Cell-Based Assays Using iPSCs for Drug Development and Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1994))

Abstract

FLIPR-based calcium assay enables the detection and characterization of neuronal excitability by using electrical field stimulation to evoke and record action potential-driven calcium transients in induced pluripotent stem cell (iPSC)-derived cortical forebrain neurons. Here we describe high throughput measurement of neuronal excitability with a defined electrostimulation paradigm in a 384-well plate format using FLIPR.

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Author information

Authors and Affiliations

  1. CNS Diseases Research, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany

    Valeria Kizner, Sandra Fischer, Benjamin Jähnke & Maximilian Naujock

Authors
  1. Valeria Kizner
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  2. Sandra Fischer
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  3. Benjamin Jähnke
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  4. Maximilian Naujock
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Corresponding author

Correspondence to Valeria Kizner .

Editor information

Editors and Affiliations

  1. Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

    Carl-Fredrik Mandenius

  2. Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

    James A. Ross

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Kizner, V., Fischer, S., Jähnke, B., Naujock, M. (2019). Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm. In: Mandenius, CF., Ross, J. (eds) Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol 1994. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9477-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9477-9_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9476-2

  • Online ISBN: 978-1-4939-9477-9

  • eBook Packages: Springer Protocols

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