Abstract
Primary endothelial cells are needed for angiogenesis studies, and more particularly in the field of tissue engineering, to engineer pre-vascularized tissues. Investigations often use human umbilical vein endothelial cells due to their extensive characterization, but also because they are easy to obtain and isolate. An alternative is the use of human dermal microvascular endothelial cells, more representative of adult skin angiogenesis and vascularization processes. This chapter presents a detailed methodology to isolate and culture microvascular endothelial cells from skin biopsies based on enzymatic digestion and mechanical extraction.
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Acknowledgments
The authors acknowledge the precious help and expertise of Sebastien Larochelle, as well as the contribution of Adele Mauroux for the cell isolation. We acknowledge the support of the Centre de recherche du CHU de Québec – Université Laval, of the Fonds de recherche du Québec-Santé (FRQS), and of the Quebec Network for cell and tissue therapies—ThéCell (a thematic network funded by the FRQS).
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Bourland, J., Mayrand, D., Tremblay, N., Moulin, V.J., Fradette, J., Auger, F.A. (2019). Isolation and Culture of Human Dermal Microvascular Endothelial Cells. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_7
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DOI: https://doi.org/10.1007/978-1-4939-9473-1_7
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