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Skin Tissue Engineering pp 205–215Cite as

Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1993))

Abstract

This chapter is intended to be a guide to skin studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for transmission electron microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.

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References

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Acknowledgments

Many thanks to Drs. José María Mateos and Andres Kaech for critically reading of the manuscript, thoughtful comments, and many advices. Special thanks to my former colleague Dr. Bruno Guhl, who trained me with his outstanding craftsmanship about the techniques of electron microscopy. I thank the whole team of the Center for Microscopy and Image Analysis for the excellent collaboration over the years. Illustrations: University of Zurich, Information Technology, MELS/SIVIC, Nadja Baltensweiler.

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Authors and Affiliations

  1. Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland

    Gery Barmettler & Urs Ziegler

Authors
  1. Gery Barmettler
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  2. Urs Ziegler
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Corresponding author

Correspondence to Gery Barmettler .

Editor information

Editors and Affiliations

  1. University Children’s Hospital Zurich, Zurich, Switzerland

    Sophie Böttcher-Haberzeth  & Thomas Biedermann  & 

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Barmettler, G., Ziegler, U. (2019). Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9473-1_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9472-4

  • Online ISBN: 978-1-4939-9473-1

  • eBook Packages: Springer Protocols

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