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Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

The cortical microtubule and actin meshworks play a central role in the shaping of plant cells. Transgenic plants expressing fluorescent protein markers specifically tagging the two main cytoskeletal systems are available, allowing noninvasive in vivo studies. Advanced microscopy techniques, in particular confocal laser scanning microscopy (CLSM), spinning disk confocal microscopy (SDCM), and variable angle epifluorescence microscopy (VAEM), can be nowadays used for imaging the cortical cytoskeleton of living cells with unprecedented spatial and temporal resolution. With the aid of free computing tools based on the publicly available ImageJ software package, quantitative information can be extracted from microscopic images and video sequences, providing insight into both architecture and dynamics of the cortical cytoskeleton.

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Acknowledgments

The update and extension of this chapter, which was previously published as ref. 29,, has been supported by the GAČR 15-02610S project. We thank Boris Voigt, Richard Cyr and Matyáš Fendrych for transgenic Arabidopsis lines, Ondřej Šebesta, Ondřej Horváth, and Aleš Soukup for expert microscopy advice, Shiqi Zhang for helpful suggestions, and Marta Čadyová for technical assistance.

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Authors and Affiliations

  1. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic

    Amparo Rosero, Denisa Oulehlová, Viktor Žárský & Fatima Cvrčková

  2. Coordinación de Innovación Regional, C.I. Turipaná, Montería, Córdoba, Colombia

    Amparo Rosero

  3. Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic

    Denisa Oulehlová & Viktor Žárský

Authors
  1. Amparo Rosero
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  2. Denisa Oulehlová
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  3. Viktor Žárský
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  4. Fatima Cvrčková
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Correspondence to Fatima Cvrčková .

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Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Rosero, A., Oulehlová, D., Žárský, V., Cvrčková, F. (2019). Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

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