Abstract
In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.
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Acknowledgments
I acknowledge the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR) and by European Regional Development Fund-Project “National infrastructure for biological and medical imaging” (No. CZ.02.1.01/0.0/0.0/16_013/0001775) for their support.
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Nebesářová, J. (2019). Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_4
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DOI: https://doi.org/10.1007/978-1-4939-9469-4_4
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