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Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.

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Acknowledgments

The work was supported by the Czech Ministry of Education, Youth and Sports (project LO1417), Charles University Grant Agency (GAUK 1002216), and the Czech Science Foundation (projects GA16-10948S and GA16–19557S).

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Authors and Affiliations

  1. Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic

    Petr Klíma & Karel Müller

  2. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic

    Vojtěch Čermák, Miroslav Srba, Jan Petrášek, Josef Šonka, Lukáš Fischer & Zdeněk Opatrný

  3. The Czech Academy of Sciences, Institute of Experimental Botany, Prague, Czech Republic

    Jan Petrášek

Authors
  1. Petr Klíma
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  2. Vojtěch Čermák
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  3. Miroslav Srba
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  4. Karel Müller
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  5. Jan Petrášek
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  6. Josef Šonka
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  7. Lukáš Fischer
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  8. Zdeněk Opatrný
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Corresponding author

Correspondence to Zdeněk Opatrný .

Editor information

Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Klíma, P. et al. (2019). Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_25

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

  • eBook Packages: Springer Protocols

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