Abstract
Fluorescence proteins changing spectral properties after exposure to light with a specific wavelength have recently become outstanding aids in the study of intracellular protein dynamics. Herein we show using Arabidopsis SYNAPTOTAGMIN 1 as a model protein that the Dendra2 green to red photoconvertible protein tag in combination with confocal scanning laser microscopy is a useful tool to study membrane protein intracellular dynamics.
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Acknowledgments
This work has been supported by the Slovak Research and Development Agency (grant no. APVV-16-0398). J.J. thanks also the Alexander von Humboldt Foundation for supporting his renewed research stay at MPIPZ, Cologne.
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Lešková, A., Kusá, Z., Labajová, M., Krausko, M., Jásik, J. (2019). The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_13
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DOI: https://doi.org/10.1007/978-1-4939-9469-4_13
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