Abstract
Sequestration of a transcription factor in a cellular membrane and releasing it on demand is an additional layer of gene regulation that is considered a rapid mode to reprogram a gene expression cascade when a plasma membrane stress signal is perceived. Better understanding of the dynamic exchange of membrane-bound transcription factors (MTFs) during biotic stress requires the development of a simple, efficient, and quick assay system. Here we report an Agrobacterium-based transient transformation method to assay the localization of fluorescent protein-tagged MTFs in tomato leaf epidermal peels that are subsequently infected with a pathogenic fungus. Essentially, our method mimics natural infection and facilitates the realistic monitoring of MTF movement during activation of a signaling event.
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Chowdhury, S., Bhattacharjee, P., Basak, S., Chowdhury, S., Kundu, P. (2019). Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato. In: Gassmann, W. (eds) Plant Innate Immunity. Methods in Molecular Biology, vol 1991. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9458-8_7
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DOI: https://doi.org/10.1007/978-1-4939-9458-8_7
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Publisher Name: Humana, New York, NY
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