Abstract
Testing plant responses to natural sources of mechanical vibration requires methods that can precisely reproduce complex vibrational stimuli. Here we describe a method for conducting high-fidelity vibrational playbacks using consumer audio equipment and custom-written signal processing software.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Karban R (2015) Plant sensing and communication. University of Chicago Press, Chicago, IL
Gagliano M, Mancuso S, Robert D (2012) Toward understanding plant bioacoustics. Trends Plant Sci 17:323–325
Gagliano M (2013) Green symphonies: a call for studies on acoustic communication in plants. Behav Ecol 24:7
Bailey NW, Fowler-Finn KD, Rebar D et al (2013) Green symphonies or wind in the willows? Testing acoustic communication in plants. Behav Ecol 24:797–798
Appel HM, Cocroft RB (2014) Plants respond to leaf vibrations caused by insect herbivore chewing. Oecologia 175:1257–1266
Cocroft RB, Appel HA (2013) Comments on ‘Green symphonies’. Behav Ecol 24:800
Schoner MG, Simon R, Schoner CR (2016) Acoustic communication in plant-animal interactions. Curr Opin Plant Biol 32:88–95
De Luca PA, Vallejo-Marín M (2013) What’s the ‘buzz’ about? The ecology and evolutionary significance of buzz-pollination. Curr Opin Plant Biol 16:429–435
Hamant O, Haswell ES (2017) Life behind the wall: sensing mechanical cues in plants. BMC Biol 15:59
Monhausen GB, Haswell ES (2013) A force of nature: molecular mechanisms of mechanoperception in plants. J Exp Bot 64:4663–4680
Braam J (2005) In touch: plant responses to mechanical stimuli. New Phytol 165:373–389
Telewski FW (2006) A unified hypothesis of mechanoperception in plants. Am J Bot 93:1466–1476
Mishra RC, Ghosh R, Bae H (2016) Plant acoustics: in the search of a sound mechanism for sound signaling in plants. J Exp Bot 67:4483–4494
Ghosh R, Gururani MA, Ponpandian LN et al (2017) Expression analysis of sound vibration-regulated genes by touch treatment in Arabidopsis. Front Plant Sci 8:100
Braam J, Davis RW (1990) Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis. Cell 60:357–364
Jung J, Kim SK, Kim JY et al (2018) Beyond chemical triggers: evidence for sound-evoked physiological reactions in plants. Front Plant Sci 30:9–25
Fernandez-Jaramillo AA, Duarte-Galvan C, Garcia-Mier L et al (2018) Effects of acoustic waves on plants: an agricultural, ecological, molecular and biochemical perspective. Sci Hortic 235:340–348
Ghosh R, Mishra RC, Choi B et al (2016) Exposure to sound vibrations lead to transcriptomic, proteomic and hormonal changes in Arabidopsis. Sci Rep 6:33370
Lopez-Ribera I, Vicient CM (2017) Drought tolerance induced by sound in Arabidopsis plants. Plant Signal Behav 12:e1368938
Cocroft RB, Rodriguez RL (2005) The behavioral ecology of vibrational communication in insects. Bioscience 55:323–334
Cocroft RB, Hamel J, Su Q, Gibson J (2014) Vibrational playback experiments: challenges and solutions. In: Cocroft RB, Gogala M, PSM H, Wessel A (eds) Studying vibrational communication. Springer, Berlin, Heidelberg
Rebar D, Hobel G, Rodriguez RL (2012) Vibrational playback by means of airborne stimuli. J Exp Biol 215:3513–3518
Hill PSM, Wessel A (2016) Biotremology. Curr Biol 26:R187–R191
Acknowledgments
We thank Alexis Kollasch, Ashten Kimble, and Barb Sonderman for help with figures and logistics, Jon Patman for troubleshooting hardware-software connections, Walter Gassman and Sharon Pike for comments on the manuscript, and Carlos Pinto Navia and the Appel lab for testing the Matlab code. National Science Foundation grant IOS-1359593 to HMA and RBC supported this work.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Michael, S.C.J., Appel, H.A., Cocroft, R.B. (2019). Methods for Replicating Leaf Vibrations Induced by Insect Herbivores. In: Gassmann, W. (eds) Plant Innate Immunity. Methods in Molecular Biology, vol 1991. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9458-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9458-8_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9457-1
Online ISBN: 978-1-4939-9458-8
eBook Packages: Springer Protocols