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Plant Innate Immunity pp 101–106Cite as

Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1991))

Abstract

Cell ploidy levels are regulated by developmental and environmental factors and they also impact the outcome of plant microbe interactions. Here we describe a simple and quick procedure to measure cell ploidy levels in Arabidopsis thaliana leaves by flow cytometry. Cell nuclei are isolated by filtering tissue homogenates from chopped plant tissues. DNA in the nuclei is stained by propidium iodide and the fluorescence emitted from the DNA of each nucleus is read by using a flow cytometer. Distribution of ploidy levels within the plant tissues can be calculated based on the distribution of fluorescence signals. Multiple samples can be prepared and analyzed within the same day.

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Acknowledgments

The work in J. Hua’s lab is supported by National Science Foundation IOS-1353738 to J.H.

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Authors and Affiliations

  1. Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA

    Leiyun Yang, Zhixue Wang & Jian Hua

  2. State Key Laboratory of Rice Biology, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, China

    Zhixue Wang

Authors
  1. Leiyun Yang
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  2. Zhixue Wang
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  3. Jian Hua
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Corresponding author

Correspondence to Jian Hua .

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Editors and Affiliations

  1. Division of Plant Sciences, Christopher S. Bond Life Sciences Center, and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, USA

    Walter Gassmann

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Yang, L., Wang, Z., Hua, J. (2019). Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry. In: Gassmann, W. (eds) Plant Innate Immunity. Methods in Molecular Biology, vol 1991. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9458-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9458-8_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9457-1

  • Online ISBN: 978-1-4939-9458-8

  • eBook Packages: Springer Protocols

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