Abstract
Signal interference or overlap in mass cytometry is minimal compared to flow cytometry but must still be considered for optimal panel design and assay sensitivity. Here we describe a procedure for evaluating signal interference dynamics in the context of a 25-parameter core immunophenotyping panel. Specifically, a mass-minus-many (MMM) approach was used to assess background signals in “empty” or “blank” channels intended for further customization. Through this approach cell type-specific variability in signal background is revealed. Further panel customization can thus be performed with an understanding of cell type and channel-specific background levels to enable rational panel design and the objective delineation of gating thresholds during analysis.
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Au-Yeung, A., Takahashi, C., Mathews, W.R., O’Gorman, W.E. (2019). Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating. In: McGuire, H., Ashhurst, T. (eds) Mass Cytometry. Methods in Molecular Biology, vol 1989. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9454-0_3
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DOI: https://doi.org/10.1007/978-1-4939-9454-0_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9453-3
Online ISBN: 978-1-4939-9454-0
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