Abstract
Mass cytometry is a technique that uses inductively coupled plasma mass spectrometry (ICP-MS) to quantify the isotopic composition of cells in suspension. Traditionally it has been used in conjunction with antibodies labeled with stable lanthanide isotopes to investigate cellular heterogeneity. Here we describe its use to quantify uptake of metal nanoparticles by cells in suspension.
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Change history
19 December 2019
This chapter was inadvertently published with the acknowledgement section leaving out the following sentence: “This work received funding from South Australian Government PRIF program Project “International Cluster on Nanosafety” of Nicolas H. Voelcker and Enzo Lombi.” This correction has been updated in the chapter.
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Acknowledgments
The authors acknowledge Hannah Kelly for providing gold nanoparticles. Funding from Future Industries Institute of the University of South Australia (Foundation Fellowship of Ivask) and Estonian Research Council grant PUT748 is acknowledged. This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at the University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF).This work received funding from South Australian Government PRIF program Project “International Cluster on Nanosafety” of Nicolas H. Voelcker and Enzo Lombi.
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Mitchell, A.J., Ivask, A., Ju, Y. (2019). Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry. In: McGuire, H., Ashhurst, T. (eds) Mass Cytometry. Methods in Molecular Biology, vol 1989. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9454-0_15
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DOI: https://doi.org/10.1007/978-1-4939-9454-0_15
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