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Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry

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Mass Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1989))

Abstract

Mass cytometry is a multi-parametric technique that offers insight into functional and biological systems at a single cell level (Tanner et al., Cancer Immunol Immunother 62:955–965, 2013). One of the major advantages of mass cytometry is the ability to measure multiple intracellular markers, including phosphorylated proteins that are part of major signaling pathways, such as NF-κB, JAK/STAT, and ERK/MAPK. Here we describe an optimized mass cytometry protocol for staining human clinical blood samples with panels that include phosphorylated antibodies.

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References

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Author information

Authors and Affiliations

  1. Discipline of Pharmacology, School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia

    Diana Shinko & Kellie A. Charles

  2. Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, NSW, Australia

    Thomas M. Ashhurst

  3. Viral Immunopathology Laboratory, Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia

    Thomas M. Ashhurst

  4. Ramaciotti Facility for Human Systems Biology (RFHSB), The University of Sydney and Centenary Institute, Sydney, NSW, Australia

    Thomas M. Ashhurst

  5. Marie Bashir Institute for Emerging Infectious Disease (MBI), The University of Sydney, Sydney, NSW, Australia

    Thomas M. Ashhurst

  6. Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, NSW, Australia

    Helen M. McGuire

  7. Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia

    Helen M. McGuire

  8. Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia

    Helen M. McGuire

Authors
  1. Diana Shinko
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  2. Thomas M. Ashhurst
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  3. Helen M. McGuire
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  4. Kellie A. Charles
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Corresponding author

Correspondence to Kellie A. Charles .

Editor information

Editors and Affiliations

  1. Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia

    Helen M. McGuire

  2. Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, NSW, Australia

    Thomas M. Ashhurst

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Shinko, D., Ashhurst, T.M., McGuire, H.M., Charles, K.A. (2019). Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry. In: McGuire, H., Ashhurst, T. (eds) Mass Cytometry. Methods in Molecular Biology, vol 1989. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9454-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9454-0_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9453-3

  • Online ISBN: 978-1-4939-9454-0

  • eBook Packages: Springer Protocols

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