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Antigen Processing pp 375–386Cite as

Identification of Human Antigen-Specific CD4+ T-Cells with Peptide–MHC Multimer Technologies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1988))

Abstract

The development of peptide–MHC class II multimers has provided a novel approach in studying antigen-specific CD4+ T-cells and extended the knowledge of these T cells in various disease settings, including infectious diseases, autoimmune diseases, cancer, and allergies. This chapter discusses the various applications of the peptide–MHC class II multimer technologies, specifically their uses in the evaluation of antigen-specific CD4+ T-cells ex vivo, and their uses in epitope identification.

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References

  1. Davis MM et al (1998) Ligand recognition by alpha beta T cell receptors. Annu Rev Immunol 16:523–544

    Article  CAS  Google Scholar 

  2. Wooldridge L et al (2009) Tricks with tetramers: how to get the most from multimeric peptide-MHC. Immunology 126(2):147–164

    Article  CAS  Google Scholar 

  3. Altman JD et al (1996) Phenotypic analysis of antigen-specific T lymphocytes. Science 274(5284):94–96

    Article  CAS  Google Scholar 

  4. Crawford F, Kozono H, White J, Marrack P, Kappler J (1998) Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes. Immunity 8(6):675–682

    Article  CAS  Google Scholar 

  5. Novak EJ, Liu AW, Nepom GT, Kwok WW (1999) MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen. J Clin Invest 104(12):R63–R67

    Article  CAS  Google Scholar 

  6. Uchtenhagen H et al (2016) Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining. Nat Commun 7:12614

    Article  CAS  Google Scholar 

  7. Newell EW, Sigal N, Bendall SC, Nolan GP, Davis MM (2012) Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity 36(1):142–152

    Article  CAS  Google Scholar 

  8. Newell EW et al (2013) Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization. Nat Biotechnol 31(7):623–629

    Article  CAS  Google Scholar 

  9. Wu X et al (2017) Lanthanide-coordinated semiconducting polymer dots used for flow cytometry and mass cytometry. Angew Chem Int Ed Engl 56(47):14908–14912

    Article  CAS  Google Scholar 

  10. Batard P et al (2006) Dextramers: new generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells. J Immunol Methods 310(1–2):136–148

    Article  CAS  Google Scholar 

  11. Huang J et al (2016) Detection, phenotyping, and quantification of antigen-specific T cells using a peptide-MHC dodecamer. Proc Natl Acad Sci U S A 113(13):E1890–E1897

    Article  CAS  Google Scholar 

  12. Bethune MT, Comin-Anduix B, Hwang Fu YH, Ribas A, Baltimore D (2017) Preparation of peptide-MHC and T-cell receptor dextramers by biotinylated dextran doping. BioTechniques 62(3):123–130

    Article  CAS  Google Scholar 

  13. Lissina A et al (2009) Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers. J Immunol Methods 340(1):11–24

    Article  CAS  Google Scholar 

  14. James EA et al (2007) Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition. Int Immunol 19(11):1291–1301

    Article  CAS  Google Scholar 

  15. Novak EJ et al (2001) Tetramer-guided epitope mapping: rapid identification and characterization of immunodominant CD4+ T cell epitopes from complex antigens. J Immunol 166(11):6665–6670

    Article  CAS  Google Scholar 

  16. Reijonen H, Kwok WW (2003) Use of HLA class II tetramers in tracking antigen-specific T cells and mapping T-cell epitopes. Methods 29(3):282–288

    Article  CAS  Google Scholar 

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Acknowledgments

Supported by US National Institutes of Health contract HHSN272201400049C.

Disclosure of potential conflict of interest: Y. Y. Kong and W. W. Kwok have received research support from the US National Institutes of Health.

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Authors and Affiliations

  1. Benaroya Research Institute at Virginia Mason, Seattle, WA, USA

    Ying Ying Kong & William W. Kwok

  2. Department of Medicine, University of Washington, Seattle, WA, USA

    William W. Kwok

Authors
  1. Ying Ying Kong
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  2. William W. Kwok
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Corresponding author

Correspondence to William W. Kwok .

Editor information

Editors and Affiliations

  1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

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Kong, Y.Y., Kwok, W.W. (2019). Identification of Human Antigen-Specific CD4+ T-Cells with Peptide–MHC Multimer Technologies. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_26

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  • DOI: https://doi.org/10.1007/978-1-4939-9450-2_26

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9449-6

  • Online ISBN: 978-1-4939-9450-2

  • eBook Packages: Springer Protocols

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