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Evaluating CD8+ T Cell Responses In Vitro

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Antigen Processing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1988))

Abstract

The 51Cr-release assay described in the 1960s has been for decades the gold standard cytolytic assay but for safety reasons is no longer in use in many laboratories. Whereas other radioactive tests were later on described, they never ousted the 51Cr-release assay. More thorough understanding of CTL biology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8+ T cell responses. Allowing for real-time evaluation of target cell viability, some of them are particularly attractive and are described here together with traditional assays.

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Acknowledgments

This work was supported by ANR grant HLA (ANR-10-BIOT-008). I thank Dr. Steve Pascolo for careful reading of the manuscript.

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Authors and Affiliations

  1. INSERM U1016, Institute Cochin, Paris, France

    François A. Lemonnier

  2. Faculté de Médecine René Descartes, Université Paris Descartes, Paris, France

    François A. Lemonnier

Authors
  1. François A. Lemonnier
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Corresponding author

Correspondence to François A. Lemonnier .

Editor information

Editors and Affiliations

  1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

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Lemonnier, F.A. (2019). Evaluating CD8+ T Cell Responses In Vitro. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9450-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9449-6

  • Online ISBN: 978-1-4939-9450-2

  • eBook Packages: Springer Protocols

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