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Radiation Cytogenetics pp 75–85Cite as

Nuclear Foci Assays in Live Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1984))

Abstract

DNA double strand breaks (DSBs) are a serious threat to genome stability and cell viability. Accurate detection of DSBs is critical for the basic understanding of cellular response to ionizing radiation. Recruitment and retention of DNA repair and response proteins at DSBs can be conveniently visualized by fluorescence imaging (often called ionizing radiation-induced foci) both in live and fixed cells. In this chapter, we describe a live cell imaging methodology that directly monitors induction and repair of single DSB, recruitment kinetics of DSB repair/sensor factors to DSB sites, and dynamic interaction of DSB repair/sensor proteins with DSBs at single-cell level. Additionally, the methodology described in this chapter can be readily adapted to other DSBs repair/sensor factors and cell types.

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Acknowledgment

This work was supported by the National Institute of Aging (R01AG053341) Grant (to A.A.).

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Authors and Affiliations

  1. Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Eiichiro Mori & Aroumougame Asaithamby

Authors
  1. Eiichiro Mori
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  2. Aroumougame Asaithamby
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Corresponding author

Correspondence to Aroumougame Asaithamby .

Editor information

Editors and Affiliations

  1. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA

    Takamitsu A. Kato

  2. Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA

    Paul F. Wilson

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Mori, E., Asaithamby, A. (2019). Nuclear Foci Assays in Live Cells. In: Kato, T., Wilson, P. (eds) Radiation Cytogenetics. Methods in Molecular Biology, vol 1984. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9432-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9432-8_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9430-4

  • Online ISBN: 978-1-4939-9432-8

  • eBook Packages: Springer Protocols

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