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PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations

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Radiation Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1984))

Abstract

Dicentric and centric ring chromosomes are used for radiation-induced damage analysis and biodosimetry after radiation exposure. However, Giemsa stain-based cytogenetic analysis is labor-intense and time-consuming. Moreover, the disadvantage of Giemsa based chromosome analysis is a potential poor reproducibility when researchers are not fully trained for analysis. These problems come from analysis of morphological abnormality of chromosomal aberrations. Locus-specific FISH probes were used to overcome this problem. Centromere probes can visualize centromere locations and help identify dicentric chromosomes and centric rings. Telomere probes help to identify terminal deletion and telomere fusions. Probes were originally designed with a DNA probe but Peptide nucleic acid (PNA) probes took the place of DNA probes. This chapter introduces PNA telomere and centromere FISH staining and accurate analysis of chromosomal aberrations.

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Author information

Authors and Affiliations

  1. Department of Medicine, University of Colorado Denver, Aurora, CO, USA

    Ian M. Cartwright

  2. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA

    Jeremy S. Haskins & Takamitsu A. Kato

Authors
  1. Ian M. Cartwright
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  2. Jeremy S. Haskins
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  3. Takamitsu A. Kato
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Corresponding author

Correspondence to Ian M. Cartwright .

Editor information

Editors and Affiliations

  1. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA

    Takamitsu A. Kato

  2. Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA

    Paul F. Wilson

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Cartwright, I.M., Haskins, J.S., Kato, T.A. (2019). PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations. In: Kato, T., Wilson, P. (eds) Radiation Cytogenetics. Methods in Molecular Biology, vol 1984. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9432-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9432-8_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9430-4

  • Online ISBN: 978-1-4939-9432-8

  • eBook Packages: Springer Protocols

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