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Proteomic Methods to Evaluate NOX-Mediated Redox Signaling

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NADPH Oxidases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1982))

Abstract

The NADPH oxidase (NOX) family of proteins is involved in regulating many diverse cellular processes, which is largely mediated by NOX-mediated reversible oxidation of target proteins in a process known as redox signaling. Protein cysteine residues are the most prominent targets in redox signaling, and to understand the mechanisms by which NOX affect cellular pathways, specific methodology is required to detect specific oxidative cysteine modifications and to identify targeted proteins. Among the many potential redox modifications involving cysteine residues, reversible modifications most relevant to NOX are sulfenylation (P-SOH) and S-glutathionylation (P-SSG), as both can induce structural or functional alterations. Various experimental approaches have been developed to detect these specific modifications, and this chapter will detail state-of-the-art methodology to selectively evaluate these modifications in specific target proteins in relation to NOX activation. We also discuss some of the limitations of these procedures and potential complementary approaches.

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Acknowledgments

The authors gratefully acknowledge research support from NHLBI and NIA (grants R01 HL085646, R01 HL138708 and R21 AG055325), as well as Fellowship support from NIH (T32 HL076122 and F31 HL142221).

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, College of Medicine, University of Vermont, Burlington, VT, USA

    Christopher M. Dustin, Milena Hristova, Caspar Schiffers & Albert van der Vliet

Authors
  1. Christopher M. Dustin
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  2. Milena Hristova
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  3. Caspar Schiffers
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  4. Albert van der Vliet
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Corresponding author

Correspondence to Albert van der Vliet .

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Editors and Affiliations

  1. Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland

    Ulla G. Knaus

  2. Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Thomas L. Leto

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Dustin, C.M., Hristova, M., Schiffers, C., van der Vliet, A. (2019). Proteomic Methods to Evaluate NOX-Mediated Redox Signaling. In: Knaus, U., Leto, T. (eds) NADPH Oxidases. Methods in Molecular Biology, vol 1982. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9424-3_30

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  • DOI: https://doi.org/10.1007/978-1-4939-9424-3_30

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9423-6

  • Online ISBN: 978-1-4939-9424-3

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