Abstract
A growing appreciation of NADPH oxidases (NOXs) as mediators of fundamental physiological processes and as important players in myriad diseases has led many laboratories on a search for specific inhibitors to help dissect the role in a given pathway or pathological condition. To date, there are only a few available inhibitors with a demonstrated specificity for a given isozyme. Among those, peptidic inhibitors have the advantage of being designed to target very specific protein-protein interactions that are essential for NOX activity. Herein, we provide the techniques to deliver these inhibitors both in cell culture as well as in vivo.
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References
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Acknowledgments
Research in the Pagano Lab is supported by the National Institutes of Health Grants R01HL079207 and P01HL103455-01 and receives support from the Institute for Transfusion Medicine and the Hemophilia Center of Western Pennsylvania.
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Cifuentes-Pagano, E., Pagano, P.J. (2019). Rational Design and Delivery of NOX-Inhibitory Peptides. In: Knaus, U., Leto, T. (eds) NADPH Oxidases. Methods in Molecular Biology, vol 1982. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9424-3_24
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DOI: https://doi.org/10.1007/978-1-4939-9424-3_24
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