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In Ovo Electroporation of Plasmid DNA and Morpholinos into Specific Tissues During Early Embryogenesis

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Neural Crest Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1976))

Abstract

In ovo electroporation enables transfection of non-viral plasmid DNA and/or morpholinos to fluorescently label and/or perturb gene function in cells of interest. However, targeted electroporation into specific subregions of the embryo can be challenging due to placement and size limitations of the electrodes. Here we describe the basic techniques for in ovo electroporation in the chick embryo and suggest parameters to electroporate cells within different target tissues that with some modifications may be applicable to a wide range of developmental stages and other embryo model organisms.

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Author information

Authors and Affiliations

  1. Stowers Institute for Medical Research, Kansas City, MO, USA

    Rebecca McLennan & Paul M. Kulesa

  2. Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, KS, USA

    Paul M. Kulesa

Authors
  1. Rebecca McLennan
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  2. Paul M. Kulesa
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Corresponding author

Correspondence to Rebecca McLennan .

Editor information

Editors and Affiliations

  1. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Quenten Schwarz

  2. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Sophie Wiszniak

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McLennan, R., Kulesa, P.M. (2019). In Ovo Electroporation of Plasmid DNA and Morpholinos into Specific Tissues During Early Embryogenesis. In: Schwarz, Q., Wiszniak, S. (eds) Neural Crest Cells. Methods in Molecular Biology, vol 1976. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9412-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9412-0_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9411-3

  • Online ISBN: 978-1-4939-9412-0

  • eBook Packages: Springer Protocols

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