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Single Cell Methods pp 235–250Cite as

Studying DNA Methylation in Single-Cell Format with scBS-seq

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1979))

Abstract

DNA methylation at cytosine is a major epigenetic mark, heavily implicated in controlling key cellular processes such as development and differentiation, cellular memory, or carcinogenesis. Bisulfite treatment in conjunction with next generation sequencing has been a powerful tool for studying this modification in a quantitative manner in the context of the whole genome and with a single nucleotide resolution. This chapter describes a protocol for bisulfite sequencing adapted to a single-cell format that allows for capturing the methylation signal from up to 50% CpG nucleotides in each cell.

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Author information

Authors and Affiliations

  1. Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK

    Natalia Kunowska

Authors
  1. Natalia Kunowska
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Corresponding author

Correspondence to Natalia Kunowska .

Editor information

Editors and Affiliations

  1. Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy

    Valentina Proserpio

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Kunowska, N. (2019). Studying DNA Methylation in Single-Cell Format with scBS-seq. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9240-9_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9239-3

  • Online ISBN: 978-1-4939-9240-9

  • eBook Packages: Springer Protocols

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