Abstract
Peripheral blood mononuclear cells (PBMCs) are blood cells that are a critical part of the immune system used to fight off infection. However, due to the complexity of PBMCs, which contain multiple different cell types, studying the function of the individual cell types can be difficult, and often studies rely on bulk measurements. Here, we describe the analysis of PBMCs using single-cell RNA-sequencing in droplets. Data from these studies allow for the identification and quantification of the subpopulation of cells that make up the PBMC sample. In addition, differential gene expression between cell types and samples can be assessed.
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Acknowledgments
This work was supported by the University of Texas Southwestern Medical Center Genomics Core. We would like to thank Gunjan Choudhary for her careful reading and editing of this book chapter.
Bio-Rad, Droplet Digital PCR, and ddPCR are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owners.
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Khan, S., Kaihara, K.A. (2019). Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_10
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DOI: https://doi.org/10.1007/978-1-4939-9240-9_10
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9240-9
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