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Personalized Metabolomics

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High-Throughput Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1978))

Abstract

The human metabolome is the cumulative product of ingested metabolites and those produced by the body and its microbiota. Together these metabolites can dynamically report on the health and disease state of an individual, as well as their response to drug treatments and other external perturbations. Profiling metabolites in human body fluids provides an opportunity to identify biomarkers and stratify patients for personalized treatments but requires the development of high-throughput approaches compatible with large cohort and longitudinal studies. Here we review in detail sample preparation and analytical liquid chromatography-mass spectrometry (LC-MS) methods to measure the broad chemical diversity of metabolites found in human plasma and urine.

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Acknowledgment

This work was supported by NIH grant F32 HL 132452 to D.P.M.

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Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA

    David P. Marciano & Michael P. Snyder

Authors
  1. David P. Marciano
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  2. Michael P. Snyder
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Corresponding author

Correspondence to Michael P. Snyder .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA

    Angelo D'Alessandro

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Marciano, D.P., Snyder, M.P. (2019). Personalized Metabolomics. In: D'Alessandro, A. (eds) High-Throughput Metabolomics. Methods in Molecular Biology, vol 1978. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9236-2_27

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  • DOI: https://doi.org/10.1007/978-1-4939-9236-2_27

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9235-5

  • Online ISBN: 978-1-4939-9236-2

  • eBook Packages: Springer Protocols

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