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High-Throughput Metabolomics pp 369–387Cite as

Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1978))

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is an established analytical platform for analyzing metabolic profiles of cells, tissues, and body fluids. There are several advantages in introducing an NMR-based study design into metabolomics studies, including a fast and comprehensive detection, characterization, and quantification of dozens of endogenous metabolites in a single NMR spectrum. Quantitative proton 1H-NMR is the most useful NMR-based platform for metabolomics. The frozen tissues can be analyzed noninvasively using a high-resolution magic angle spinning (HR-MAS) 1H-NMR spectroscopy; or several extraction techniques can be applied to detect additional metabolites using a conventional liquid-based NMR technique. In this chapter, we report on tissue collection, handling, extraction methods, and 1H-NMR acquisition protocols developed in the past decades for a precise and quantitative NMR-metabolomics approach. The NMR acquisition protocols (both HR-MAS and conventional 1H-NMR spectroscopy) and spectral analysis steps are also presented. Since NMR can be applied “in vivo” using horizontal bore MRI scanners, several in vivo sequences for localized 1H-MRS (magnetic resonance spectroscopy) are presented which can be directly applied for noninvasive detection of brain metabolites.

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Acknowledgment

This work was supported by the NIH grants S10 OD023485, R01 CA140368, R01 CA195708, P30 CA046934, and UL1 TR001082, the University of Colorado Cancer Center (UCCC), and the Colorado Clinical Translational Sciences Institute (CCTSI) Animal Imaging Shared Resource (AISR).

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Authors and Affiliations

  1. Department of Radiology, School of Medicine, University of Colorado Denver, Aurora, CO, USA

    Natalie J. Serkova, Denise M. Davis & Jenna Steiner

  2. Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, USA

    Rajesh Agarwal

Authors
  1. Natalie J. Serkova
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  2. Denise M. Davis
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  3. Jenna Steiner
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  4. Rajesh Agarwal
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Corresponding author

Correspondence to Natalie J. Serkova .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA

    Angelo D'Alessandro

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Serkova, N.J., Davis, D.M., Steiner, J., Agarwal, R. (2019). Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy. In: D'Alessandro, A. (eds) High-Throughput Metabolomics. Methods in Molecular Biology, vol 1978. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9236-2_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9236-2_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9235-5

  • Online ISBN: 978-1-4939-9236-2

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