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Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling

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High-Throughput Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1978))

Abstract

Arginine metabolism is linked to several important metabolic processes, and reprogramming of arginine metabolism occurs in various physiological and pathological conditions. Here we describe a method, using a LC-MS-based metabolomics and 15N4-arginine tracing approach, to quantitatively analyze arginine metabolism. This method can reliably quantify the abundance of important intermediates and fluxes of major metabolic reactions in arginine metabolism in a variety of cultured mammalian cell models.

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Author information

Authors and Affiliations

  1. Morgridge Institute for Research, Madison, WI, USA

    Gretchen L. Seim, Emily C. Britt & Jing Fan

  2. Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA

    Gretchen L. Seim, Emily C. Britt & Jing Fan

Authors
  1. Gretchen L. Seim
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  2. Emily C. Britt
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  3. Jing Fan
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Corresponding author

Correspondence to Jing Fan .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA

    Angelo D'Alessandro

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Seim, G.L., Britt, E.C., Fan, J. (2019). Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling. In: D'Alessandro, A. (eds) High-Throughput Metabolomics. Methods in Molecular Biology, vol 1978. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9236-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9236-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9235-5

  • Online ISBN: 978-1-4939-9236-2

  • eBook Packages: Springer Protocols

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