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RNA Interference and Cancer Therapy pp 83–98Cite as

Combinatorial siRNA Polyplexes for Receptor Targeting

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1974))

Abstract

As synthetic small interfering RNA (siRNA) against antitumoral gene targets show promise for cancer treatment, different siRNA delivery systems have sparkled intense investigations. To develop tumor-specific carriers for cytosolic and systemic siRNA delivery, our laboratory has recently generated folate-conjugated targeted combinatorial siRNA polyplexes based on sequence-defined oligomer platform compatible with solid-phase-supported synthesis. These polyplexes presented efficient siRNA-mediated gene silencing in folate receptor-expressing tumors in vitro and in vivo. In this chapter, we provide a brief background on the formulation design and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and receptor-directed cell killing in cancer cells using targeted combinatorial siRNA polyplexes.

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Acknowledgments

The work presented in this article was supported by German Research Foundation (DFG) grant SFB1032 (Project B4) and German Excellence Cluster Nanosystems Initiative Munich.

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Authors and Affiliations

  1. Department of Pharmacy, Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany

    Dian-Jang Lee & Ernst Wagner

  2. Nanosystems Initiative Munich (NIM), Munich, Germany

    Dian-Jang Lee & Ernst Wagner

Authors
  1. Dian-Jang Lee
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  2. Ernst Wagner
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Corresponding author

Correspondence to Ernst Wagner .

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Editors and Affiliations

  1. Cancer Biology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, Telangana, India

    Lekha Dinesh Kumar

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Lee, DJ., Wagner, E. (2019). Combinatorial siRNA Polyplexes for Receptor Targeting. In: Dinesh Kumar, L. (eds) RNA Interference and Cancer Therapy. Methods in Molecular Biology, vol 1974. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9220-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9220-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9219-5

  • Online ISBN: 978-1-4939-9220-1

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