The PRDI-BF1 and RIZ (PR) domain zinc finger protein 14 (PRDM14) is upregulated in approximately 60% of breast cancers, some of which exhibit gene amplification. In contrast, PRDM14 is not expressed in normal, and differentiated tissues. PRDM14+ breast cancer cells are resistant to chemotherapy drugs, are tumorigenic, and metastasize to the lungs. It is commonly assumed that genes that are overexpressed in cancers, such as PRDM14, are effective targets for new therapies that specifically abrogate the expression of these genes. RNA interference of PRDM14, a gene expressed by breast cancer cells, reduced the size of tumors and lung metastases in nude mice. In this chapter, we introduce the concept and methods to develop and apply systematically injected small interfering RNA therapy for breast cancer models in vivo.
PRDM14 Breast cancer Metastasis siRNA therapy Chimera siRNA Drug delivery systems
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This work was supported by the Department of Research Promotion, Practical Research for Innovative Cancer Control, Ministry of Health, Labour and Welfare, and the Japan Agency for Medical Research and Development (AMED). We wish to thank Prof. Kazunori Kataoka from the Innovation Center of NanoMedicine for the DDSs and Prof. Yukikazu Natori and Associate professor Kumiko Ui-Tei from the University of Tokyo for chimera siRNA and selection of highly effective siRNA sequences for RNAi. We also would like to thank Editage (www.editage.jp) for English language editing.
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