Abstract
Small interfering RNA (siRNA) is a promising tool for gene therapy-based disease treatments. However, delivery of siRNA to the target cells requires a specific and reliable carrier system. Herein we describe a targeted carrier system that can deliver siRNA to cancer cells overexpressing the human epidermal growth factor 2 (HER2) receptor. Trastuzumab-conjugated poly(amido)amine dendrimers can be synthesized using the protocols described here.
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References
Xin Y, Huang M, Guo MM et al (2017) Nano-based delivery of RNAi in cancer therapy. Mol Cancer 16:134
Fellmann C, Lowe SW (2014) Stable RNA interference rules for silencing. Nat Cell Biol 16(1):10–18
Scherman D, Rousseau A, Bigey P et al (2017) Genetic pharmacology: progresses in siRNA delivery and therapeutic applications. Gene Ther 24:151–156
Haussecker D (2014) Current issues of RNAi therapeutics delivery and development. J Control Release 195:49–54
Mizrahy S, Hazan-Halevy I, Dammes N et al (2017) Current progress in non-viral RNAi-based delivery strategies to lymphocytes. Mol Ther 25:1–10
Wang J, Lu Z, Wientjes MG et al (2010) Delivery of siRNA therapeutics: barriers and carriers. AAPS J 12:492–503
Ozpolat B, Sood AK, Lopez-Berestein G (2014) Liposomal siRNA nanocarriers for cancer therapy. Adv Drug Deliv Rev 66:110–116
Wu SY, Lopez-Berestein G, Calin GA et al (2014) RNAi therapies: drugging the undruggable. Sci Transl Med 6(240):240ps7
Yang J, Zhang Q, Chang H, Cheng Y (2015) Surface-engineered dendrimers in gene delivery. Chem Rev 115:5274–5300
Biswas S, Torchilin VP (2013) Dendrimers for siRNA delivery. Pharmaceuticals 6:161–183
Zhou J, Wu J, Hafdi N et al (2006) PAMAM dendrimers for efficient siRNA delivery and potent gene silencing. Chem Commun (Camb) 22:2362–2364
Senkusa E, Cardosob F, Pagani O (2014) Time for more optimism in metastatic breast cancer? Cancer Treat Rev 40:220–228
Sampathkumar SG, Yarema KJ (2007) Dendrimers in cancer treatment and diagnosis. In: Kumar CSSR (ed) Nanotechnologies for the life sciences, vol 7. Wiley-VCH, Weinheim
Figueroa-Magalhães MC, Jelovac D, Connolly RM, Wolff AC (2014) Treatment of HER2-positive breast cancer. Breast 23:128–136
Kulhari H, Pooja D, Rompicharla SVK et al (2015) Biomedical applications of Trastuzumab: as a therapeutic agent and a targeting ligand. Med Res Rev 35:849–876
Kulhari H, Pooja D, Shrivastava S et al (2015) Trastuzumab-grafted PAMAM dendrimers for the selective delivery of anticancer drugs to HER2-positive breast cancer. Sci Rep 6:23179
Acknowledgments
H.K. acknowledges the Department of Science and Technology, New Delhi, for INSPIRE Faculty Award. A.K.J. acknowledges the University Grants Commission, New Delhi, for Ph.D. scholarship.
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Kulhari, H., Jangid, A.K., Adams, D.J. (2019). Monoclonal Antibody-Conjugated Dendritic Nanostructures for siRNA Delivery. In: Dinesh Kumar, L. (eds) RNA Interference and Cancer Therapy. Methods in Molecular Biology, vol 1974. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9220-1_14
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DOI: https://doi.org/10.1007/978-1-4939-9220-1_14
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9220-1
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