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2′-C,4′-C-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability

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Non-Natural Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1973))

Abstract

This chapter describes procedures for (1) the synthesis of six 2′-C,4′-C-ethyleneoxy-bridged thymidine phosphoramidites, i.e., methylene-EoDNA-T, (R)-Me-methylene-EoDNA-T, (S)-Me-methylene-EoDNA-T, EoDNA-T, (R)-Me-EoDNA-T, and (S)-Me-EoDNA-T phosphoramidites, (2) the introduction of the phosphoramidites into oligonucleotides, (3) UV-melting experiments of the duplexes of the modified oligonucleotides and complementary RNA, and (4) nuclease degradation experiments of the modified oligonucleotides.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan

    Takashi Osawa & Yoshiyuki Hari

  2. Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan

    Satoshi Obika

Authors
  1. Takashi Osawa
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  2. Satoshi Obika
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  3. Yoshiyuki Hari
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Corresponding author

Correspondence to Yoshiyuki Hari .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA

    Nathaniel Shank

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Osawa, T., Obika, S., Hari, Y. (2019). 2′-C,4′-C-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability. In: Shank, N. (eds) Non-Natural Nucleic Acids. Methods in Molecular Biology, vol 1973. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9216-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9216-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9215-7

  • Online ISBN: 978-1-4939-9216-4

  • eBook Packages: Springer Protocols

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