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Flexible Nucleic Acids (FNAs) as Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates

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Non-Natural Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1973))

Abstract

The methodology enabling enzymatic and nonenzymatic information transfer with FNAs is described. This methodology includes the chemical synthesis of fNTPs and fN phosphoramidites, in addition to protocols for the enzymatic and nonenzymatic transfer of information.

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Acknowledgments

This work was supported by grants from the NASA Astrobiology Institute and the NASA Exobiology Program.

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Authors and Affiliations

  1. Department of Chemistry, University of California, Riverside, Riverside, CA, USA

    Maryline Chemama & Christopher Switzer

Authors
  1. Maryline Chemama
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  2. Christopher Switzer
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Corresponding author

Correspondence to Christopher Switzer .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA

    Nathaniel Shank

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Chemama, M., Switzer, C. (2019). Flexible Nucleic Acids (FNAs) as Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates. In: Shank, N. (eds) Non-Natural Nucleic Acids. Methods in Molecular Biology, vol 1973. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9216-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9216-4_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9215-7

  • Online ISBN: 978-1-4939-9216-4

  • eBook Packages: Springer Protocols

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