Abstract
Microchip electrophoresis (MCE), regarded as a miniaturized version of capillary electrophoresis (CE), has exhibited prominent advantages in terms of low sample consumption, rapid analysis times, easy operation, efficient resolution of compounds, and increased throughput. This technology has led to more research focus on analysis particularly in hospital settings for clinical diagnostics. However, since the channels in microchip are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe aptamer-based MCE assays for amplification detection of carcinoembryonic antigen (CEA) in human serum.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kimura H, Matsuzawa S, Tu CY, Kitamori T, Sawada T (1996) Ultrasensitive heterogeneous immunoassay using photothermal deflection spectroscopy. 2. Quantitation of ultratrace carcinoembryonic antigen in human sera. Anal Chem 68:3063–3067
Seker D, Kaya O, Adabag A, Necipoglu G, Baran I (2003) Role of preoperative plasma CA 15-3 and carcinoembryonic antigen levels in determining histopathologic conventional prognostic factors for breast cancer. World J Surg 27:519–521
Shen GY, Wang H, Deng T, Shen GL, Yu RQ (2005) A novel piezoelectric immunosensor for detection of carcinoembryonic antigen. Talanta 67:217–220
Liu Y, Jiang H (2006) Electroanalytical determination of carcinoembryonic antigen at a silica nanoparticles/titania sol-gel composite membrane-modified gold electrode. Electroanalysis 18:1007–1013
Pan J, Yang QW (2007) Antibody-functionalized magnetic nanoparticles for the detection of carcinoembryonic antigen using a flow-injection electrochemical device. Anal Bioanal Chem 388:279–286
Walter K, Norbert N, Jochen S, Rudolf P, Herbert H (1988) Is there any clinical relevance of serial determinations of serum carcinoembryonic antigen in small cell lung cancer patients. Cancer 62:1348–1354
Pergters J, Schmide-Gayk H, Peters B, Armbruster FP, Quentmeler A, Mathlas D (1989) lmmunoradiometric assay of carcinoembryonic antigen with use of avidin-biotin labeling. Clin Chem 35:573–576
Lin JH, Yan F, Ju HX (2004) Noncompetitive enzyme immunoassay for carcinoembryonic antigen by flow injection chemiluminescence. Clin Chim Acta 341:109–115
Yuan JL, Wang GL, Majima K, Matsumoto K (2001) Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay. Anal Chem 73:1869–1876
Dungchai W, Siangproh W, Lin JM, Chailapakul O, Lin S, Ying XT (2007) Development of a sensitive micro-magnetic chemiluminescence enzyme immunoassay for the determination of carcinoembryonic antigen. Anal Bioanal Chem 387:1965–1971
Ye F, Shi M, Huang Y, Zhao S (2010) Noncompetitive immunoassay for carcinoembryonic antigen in human serum by microchip electrophoresis for cancer diagnosis. Clin Chim Acta 411:1058–1062
Hou L, Tang Y, Xu M, Gao Z, Tang D (2014) Tyramine-based enzymatic conjugate repeats for ultrasensitive immunoassay accompanying tyramine signal amplification with enzymatic biocatalytic precipitation. Anal Chem 86:8352–8358
Ellington AD, Szostak JW (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346:818–822
Shangguan D, Li Y, Tang Z, Cao ZC, Chen HW, Mallikaratchy P, Sefah K, Yang CJ, Tan W (2006) Aptamers evolved from live cells as effective molecular probes for cancer study. Proc Natl Acad Sci U S A 103:11838–11843
Xue L, Zhou X, Xing D (2012) Sensitive and homogeneous protein detection based on target-triggered aptamer hairpin switch and nicking enzyme assisted fluorescence signal amplification. Anal Chem 84:3507–3513
Shang F, Guihen E, Glennon JD (2012) Recent advances in miniaturization-the role of microchip electrophoresis in clinical analysis. Electrophoresis 33:105–116
Nge PN, Rogers CI, Woolley AT (2013) Advances in microfluidic materials, functions, integration, and applications. Chem Rev 113:2550–2583
Yang T, Vdovenko M, Jin X, Sakharov IY, Zhao S (2014) Highly sensitive microfluidic competitive enzyme immunoassay based on chemiluminescence resonance energy transfer for the detection of neuron-specific enolase. Electrophoresis 35:2022–2028
Fredlake CP, Hert DG, Root BE, Barron AE (2008) Polymer systems designed specifically for DNA sequencing by microchip electrophoresis: a comparison with commercially available materials. Electrophoresis 29:4652–4662
Slagter-Jäger JG, Nicolette CA, Tcherepanova IY (2012) Evaluation of a microfluidics-based platform and slab electrophoresis for determination of size, integrity and quantification of in vitro transcribed RNA used as a component in therapeutic drug manufacturing. J Pharm Biomed Anal 70:657–663
Jin S, Anderson GJ, Kennedy RT (2013) Western blotting using microchip electrophoresis interfaced to a protein capture membrane. Anal Chem 85:6073–6079
Zhao S, Huang Y, Shi M, Liu YM (2009) Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection. J Chromatogr A 1216:5155–5159
Bi S, Yan Y, Yang X, Zhang S (2009) Gold nanolabels for new enhanced chemiluminescence immunoassay of alpha-fetoprotein based on magnetic beads. Chem Eur J 15:4704–4709
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zhao, S. (2019). Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen. In: Phillips, T.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 1972. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9213-3_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9213-3_18
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9212-6
Online ISBN: 978-1-4939-9213-3
eBook Packages: Springer Protocols