Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen

  • Shulin Zhao
Part of the Methods in Molecular Biology book series (MIMB, volume 1972)


Microchip electrophoresis (MCE), regarded as a miniaturized version of capillary electrophoresis (CE), has exhibited prominent advantages in terms of low sample consumption, rapid analysis times, easy operation, efficient resolution of compounds, and increased throughput. This technology has led to more research focus on analysis particularly in hospital settings for clinical diagnostics. However, since the channels in microchip are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe aptamer-based MCE assays for amplification detection of carcinoembryonic antigen (CEA) in human serum.

Key words

Microchip electrophoresis Aptamer Laser induced fluorescence detection Carcinoembryonic antigen 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shulin Zhao
    • 1
  1. 1.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and PharmacyGuangxi Normal UniversityGuilinChina

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