Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen
Microchip electrophoresis (MCE), regarded as a miniaturized version of capillary electrophoresis (CE), has exhibited prominent advantages in terms of low sample consumption, rapid analysis times, easy operation, efficient resolution of compounds, and increased throughput. This technology has led to more research focus on analysis particularly in hospital settings for clinical diagnostics. However, since the channels in microchip are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe aptamer-based MCE assays for amplification detection of carcinoembryonic antigen (CEA) in human serum.
Key wordsMicrochip electrophoresis Aptamer Laser induced fluorescence detection Carcinoembryonic antigen
- 7.Pergters J, Schmide-Gayk H, Peters B, Armbruster FP, Quentmeler A, Mathlas D (1989) lmmunoradiometric assay of carcinoembryonic antigen with use of avidin-biotin labeling. Clin Chem 35:573–576Google Scholar
- 20.Slagter-Jäger JG, Nicolette CA, Tcherepanova IY (2012) Evaluation of a microfluidics-based platform and slab electrophoresis for determination of size, integrity and quantification of in vitro transcribed RNA used as a component in therapeutic drug manufacturing. J Pharm Biomed Anal 70:657–663CrossRefGoogle Scholar