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Clinical Applications of Capillary Electrophoresis pp 235–250Cite as

Prostate Protein n-Glycosylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1972))

Abstract

DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis allows for accurate profiling of the asparagine-linked (n-) glycosylation patterns, a posttranslational modification present on many soluble and membrane proteins. This technique has been extensively tested to identify n-glycosylation patterns associated with serum proteins. Here we describe the use of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis to identify the n-glycosylation patterns of prostate proteins in urine.

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Author information

Authors and Affiliations

  1. Department of Medical Oncology, Ghent University Hospital, Ghent, Belgium

    Tijl Vermassen & Sylvie Rottey

  2. Center for Medical Biotechnology, VIB, Ghent, Belgium

    Nico Callewaert

  3. Drug Research Unit Ghent, Ghent University Hospital, Ghent, Belgium

    Tijl Vermassen & Sylvie Rottey

  4. Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium

    Joris R. Delanghe

Authors
  1. Tijl Vermassen
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  2. Nico Callewaert
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  3. Sylvie Rottey
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  4. Joris R. Delanghe
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Corresponding author

Correspondence to Joris R. Delanghe .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA

    Terry M. Phillips

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Vermassen, T., Callewaert, N., Rottey, S., Delanghe, J.R. (2019). Prostate Protein n-Glycosylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis. In: Phillips, T.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 1972. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9213-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9213-3_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9212-6

  • Online ISBN: 978-1-4939-9213-3

  • eBook Packages: Springer Protocols

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