Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis
An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.
Key wordsMicrofluidics Point-of-care diagnostics Rapid analysis Photolithography Cyclic olefin copolymer Hot embossing Laser induced fluorescence
- 1.World Health Organization (2012) Born too soon: the global action report on preterm birth. WHO, GenevaGoogle Scholar
- 2.March of Dimes (2013) Prematurity campaign: 2013 progress report. March of Dimes Foundation, White PlainsGoogle Scholar
- 7.Nielsen AV, Nielsen JB, Knob R, Sahore V, Woolley AT (2018) Separation of a panel of preterm birth biomarkers using microchip electrophoresis. Electrophoresis 39:2300–2307Google Scholar