Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis

  • Anna V. Nielsen
  • Adam T. WoolleyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1972)


An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.

Key words

Microfluidics Point-of-care diagnostics Rapid analysis Photolithography Cyclic olefin copolymer Hot embossing Laser induced fluorescence 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryBrigham Young UniversityProvoUSA

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