Leishmania pp 95-108 | Cite as

Quantitative RNA Analysis Using RNA-Seq

  • Peter J. MylerEmail author
  • Jacqueline A. McDonald
  • Pedro J. Alcolea
  • Aakash Sur
Part of the Methods in Molecular Biology book series (MIMB, volume 1971)


High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5′ end of all Leishmania (and other trypanosomatid) mRNAs.

Key words

RNA-seq Transcriptome mRNA Differential gene expression 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Peter J. Myler
    • 1
    • 2
    • 3
    Email author
  • Jacqueline A. McDonald
    • 1
  • Pedro J. Alcolea
    • 1
    • 4
  • Aakash Sur
    • 1
    • 3
  1. 1.Center for Global Infectious Disease ResearchSeattle Childrens Research InstituteSeattleUSA
  2. 2.Department of Global HealthUniversity of WashingtonSeattleUSA
  3. 3.Department of Biomedical Informatics and Medical EducationUniversity of WashingtonSeattleUSA
  4. 4.Department of Cellular and Molecular BiologyCentro de Investigaciones Biológicas (CSIC)MadridSpain

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