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Monoclonal Antibodies Against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitidis: A Platform for the Quality Control of Meningococcal Vaccines

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Book cover Neisseria meningitidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1969))

Abstract

Vaccination has reduced morbidity and mortality of many diseases that previously caused devastating epidemics and deaths globally. Vaccines as a biological product may contain microorganisms or their derivatives. This aspect together with the fact that they are administered to healthy individuals (mainly children) means that approximately 70% of vaccines development time is dedicated to quality control. Monoclonal antibodies (MAbs) have become essential analytical tools for application in ELISAs, Western and Dot blotting, immunoprecipitation, and flow cytometric assays that ensure the quality control of vaccines. The aim of this work is to present a review of the methods used to obtain a platform of MAbs against Neisseria meningitidis polysaccharide antigens to use as an analytical tool for quality control of anti-meningococcal polysaccharide (Ps) vaccines. The MAbs obtained are used in five sandwich ELISAs developed for Ps quantification. The assays showed good reproducibility and repeatability, with quantitation and detection limits below 1 ng/mL. Dot Blot, as the Identity test of the Ps vaccine, was carried out to positively identify licensed and experimental vaccines. All assays described are suitable for the screening of multiple vaccine samples and could be useful for monitoring lot-to-lot consistency and stability.

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Author information

Authors and Affiliations

  1. Monoclonal Antibodies Laboratory, Department of Biological Evaluation, Research Area, Finlay Institute of Vaccine, West Havana, Cuba

    Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez & Reinaldo Acevedo

Authors
  1. Elizabeth González
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  2. Fátima Reyes
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  3. Oscar Otero
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  4. Frank Camacho
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  5. Maribel Cuello
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  6. Fidel Ramírez
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  7. Reinaldo Acevedo
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Corresponding author

Correspondence to Reinaldo Acevedo .

Editor information

Editors and Affiliations

  1. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Kate L. Seib

  2. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Ian R. Peak

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González, E. et al. (2019). Monoclonal Antibodies Against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitidis: A Platform for the Quality Control of Meningococcal Vaccines. In: Seib, K., Peak, I. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 1969. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9202-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9202-7_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9201-0

  • Online ISBN: 978-1-4939-9202-7

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